|
KMID : 0364020130460050328
|
|
Korean Journal of Thoracic and Cardiovascular Surgery
2013 Volume.46 No. 5 p.328 ~ p.339
|
|
|
Influence of Mesenchymal Stem Cells on Cryopreserved Tracheal Allografts in Rabbits
|
|
Kim Hyun-Jo
|
|
|
Abstract
|
|
|
Background: Ischemic injury and the rejection process are the main reasons for graft failure in tracheal trans-plantation models. To enhance the acceptance, we investigated the influence of mesenchymal stem cells (MSCs) on tracheal allografts.
Methods: Extracted tracheal grafts from New Zealand white rabbits were cryopreserved for 4 weeks and orthotopically transplanted (control group A, n=8). In group B (n=8), cyclosporin A (CsA, 10 mg/kg) was injected daily into the peritoneal cavity. In group C (n=8), MSCs (1.0×107 cells/kg) from the same donor of the tracheal allograft, which had been pre-cultured for 4 weeks, were infused intravenously after transplantation. In group D (n=8), MSCs were infused and CsA was injected daily. Four weeks after transplantation, gross and histo-morphological assessments were conducted for graft necrosis, measuring the cross-sectional area of the allograft, determining the degree of epithelization, lymphocytic infiltration, and vascular regeneration.
Results: The morphologic integrity of the trachea was retained completely in all cases. The cross-sectional areas were decreased significantly in group A (p=0.018) and B (p=0.045). The degree of epithelization was enhanced (p=0.012) and the lymphocytic infiltration was decreased (p=0.048) significantly in group D compared to group A. The degree of vascular re-generation did not differ significantly in any of the groups. There were no significant correlations among epitheliza-tion, lymphocytic infiltration, and vascular regeneration.
Conclusion: The administration of MSCs with concurrent in-jections of CsA enhanced and promoted epithelization and prevented lymphocytic infiltration in tracheal allografts, al-lowing for better acceptance of the allograft.
|
|
|
KEYWORD
|
|
|
Mesenchymal stem cells, Trachea, Cryopreservation, Transplantation
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|